NGS is revolutionizing the field of genome biology, with much faster data generation, increased accuracy, and a dramatic reduction of sequencing costs. Multiple genomes can now be sequenced in parallel by a single instrument in a matter of days. In the medical field, NGS is already having an impact in genetic screening and holds great potential in oncology, given the genetic aspects of cancerous disease.
Cardiovascular disease is the leading cause of mortality in the United States. As early diagnosis is critical, interest in measurement of CVD biomarkers has increased dramatically. CVD biomarker discovery research has focused on arterial plaque related disease as conditions are prevalent, chronic and progressive and can be measured by soluble biomarkers.
Drug-induced liver injury (DILI) is an adverse event that results in withdrawal of drugs from market. As drugs may cause liver injury through various mechanisms, measuring multiple biomarkers is required to determine the potential for hepatotoxicity in early drug development.
Cytokines are polypeptides that regulate adaptive and innate immune responses. Their role in normal inflammatory response and immune cell development and activation drives involvement in a variety of diseases, often at low levels.
Up to one million DNA changes occur per cell per day, due to environmental factors and metabolic byproducts. If not repaired, the lesions in critical genes can alter normal functions of a cell. Dysfunctions in DNA damage response are implicated in many diseases. Similarly, genotoxicity describes the capacity of chemical agents to cause DNA damage, leading to problematic mutations.
Multiple sclerosis is a common cause of neurologic disability in young adults. Based on the past course of MS disease, several patterns of progression or subtypes have been described. In recent years, growing evidence suggests the value of peripheral biomarkers in the diagnosis and prognosis of CNS disorders with the hope to improve clinical outcomes.
Dysregulation of angiogenesis, a fundamental process in growth, development and tissue repair, leads to abnormal blood vessel growth involved in many common diseases and plays a significant role in cancer tumor growth and metastasis. We demonstrate the utility of the MILLIPLEX® MAP Human Angiogenesis/Growth Factor Panel , based on the Luminex® xMAP® bead-based multiplexed assay platform.
Mitochondria are unique and complex organelles that perform essential functions in many aspects of cell biology. The dominant function of mitochondria is the production of more than 90% of the cell’s energy in the form of ATP through oxidative phosphorylation.
Thousands of scientists around the world are making use of cutting edge technologies every day in order to push the boundaries of what we thought possible, furthering our understanding of the world around us. This dedicated pursuit of knowledge rests upon the shoulders of advances in the development of analytical instrumentation, specifically built to allow us to probe ever deeper into the processes governing the fundamental aspects of biological, chemical and physical systems.
In this report, apoptosis was induced in HL60 cells with daunorubicin, a DNA-intercalating agent which inhibits DNA and RNA synthesis and is used as a treatment for acute myeloid leukemia (AML). HL60 cells were exposed to a time course of daunorubucin treatment.
The ImageStream® imaging flow cytometry platform was used to study the mechanism of action of Rituximab (RTX). RTX is a therapeutic monoclonal antibody directed against CD20 for the treatment of Non-Hodgkin’s lymphoma.
Increasing the number of samples which can be run simultaneously is desirable for high content analysis and screening applications. To increase sample throughput, the fluorescent cell barcoding method previously reported by Krutzik & Nolan has been adapted for the ImageStream®.
Many assays for immune function require imaging, but immune cells present significant challenges to image-based analysis due to their rarity and the need for simultaneous multispectral immunophenotyping, making statistically robust quantification difficult.
Quantifying brain activity through optical imaging has the potential to improve the way the biomedical community treats neurological disorders and brain injuries. To accurately visualize and treat patients who have suffered a stroke, epileptic attack or traumatic brain injury, neuroscientists require precise imaging and measurements of brain activity.
Correlating levels of mRNA and corresponding proteins within cells provides more information linking gene function to phenotype than examining either alone. Separate measurements of RNA and protein merely provide information about two similar but separate cell populations. The ability to study both in individual cells leads to more physiologically relevant data, including information about cell-to-cell heterogeneity within a given sample.
Patience may be a virtue. But in a lab that’s bustling with scientists conducting meaningful biological research, excessive waiting can be downright frustrating. Such was the case leading up to 2012, when researchers at The University of Chicago Flow Cytometry Core Facility— known as UCFlow— would routinely wait as long as 16 days to be able to sort cells.