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Molecular Biology

NEXTflex™ Rapid Directional RNA-Seq Kit

June 15, 2015 4:39 pm | Bioo Scientific | Products

The NEXTflex™ Rapid Directional RNA-Seq Kit provides an easy and flexible dUTP-based solution for generating single read, paired-end and multiplexed stranded libraries from mRNA or rRNA depleted total RNA. This kit can be used to obtain "stranded" information which identifies the specific DNA strands a given RNA transcript was derived from with >99% strand specificity. Automation protocols are available for the Sciclone Workstations to offer researchers a substantial increase in productivity with consistent quality.

LISTED UNDER: Library Construction | RNA Analysis | Sequencing

NEXTflex™ Bisulfite-Seq Kit

June 15, 2015 4:35 pm | Bioo Scientific | Products

The NEXTflex™ Bisulfite-Seq Kit simplifies the library preparation of bisulfite-treated DNA with reduced representation or total bisulfite-converted DNA libraries. The kit is compatible with single, paired-end and multiplexed DNA NGS libraries on Illumina® sequencing platforms. “Enhanced Adapter Ligation Technology” is incorporated into the kit, offering improved ligation efficiency and resulting in library preps with a larger number of unique sequencing reads. For multiplexing, up to 24 unique, single-index methylated NEXTflex™ Bisulfite-Seq Barcodes can be purchased separately.

LISTED UNDER: Library Construction | Sequencing

NEXTflex™ Small RNA Sequencing Kit v2

June 12, 2015 4:46 pm | Bioo Scientific | Products

The patent pending NEXTflex™ Small RNA Sequencing Kit v2 provides an user-friendly, flexible, cost-effective solution for generating small RNA libraries with reduced bias for Illumina-compatible sequencing. The NEXTflex Small RNA-Seq Kit V2 using a pool of adapters having randomized sequences at the ligation site to overcoming ligation bias typically introduced during small RNA library prep.

LISTED UNDER: Library Construction | RNA Analysis | Sequencing
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Automated Next Generation Sequencing Sample Prep

May 18, 2015 9:55 am | Product Releases | Comments

Hamilton Robotics, as part of its co-marketing partnership with Illumina, is developing automated solutions for NGS sample preparation that reduce hands-on time, minimize operational variability, and deliver consistent results. Illumina is providing input on the protocols and assay performance.

LISTED UNDER: Automated Liquid Handling Systems | Automated Sequencers

Western Blot Detection System

April 28, 2015 10:17 am | Product Releases | Comments

Molecular Devices announced the launch of the ScanLater Western Blot Detection System. The system combines western blot membrane detection with a multimode microplate reader platform, eliminating the need to acquire a separate dedicated western blot system.

LISTED UNDER: Western Blotting

System Processes Up to 12 Western Blots Simultaneously

April 27, 2015 10:32 am | Product Releases | Comments

The BlotCycler from Precision Biosystems offers an automated system for performing western blots with high sensitivity and low background noise.

LISTED UNDER: Western Blotting

Scalable RNA-Seq Service Line

March 27, 2015 11:46 am | Product Releases | Comments

Beckman Coulter Genomics Introduces a Scalable RNA-Seq Service Line for Processing Intact and Degraded RNA Samples Expanded RNA sequencing options also captures stranded and long noncoding-RNA information.

LISTED UNDER: Microarray Contract | Sequencing

Improving Reproducibility in Real-Time PCR

March 26, 2015 10:50 am | Product Releases | Comments

INTEGRA has reported how a clinical diagnostic laboratory has, using a VIAFLO 96 handheld electronic pipette, significantly improved the reproducibility and productivity with which it carries out Real-time PCR assays.

LISTED UNDER: RT-PCR | Multi-channel Pipettors
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Digital PCR System Aids Life Science Experiments

March 24, 2015 11:40 am | Product Releases | Comments

Bio-Rad Laboratories Inc. launched the QX200 Droplet Digital PCR (ddPCR) system, the second generation of the company's ddPCR technology. The system is the only digital PCR system that works with EvaGreen and TaqMan hydrolysis probes (reagents that are used with the instrument) to provide users more flexibility in the design of their digital PCR experiments.

LISTED UNDER: DNA Amplification

Real-time PCR Dye

March 18, 2015 12:07 pm | Product Releases | Comments

Precision Blue real-time PCR dye from Bio-Rad Laboratories makes it easier for researchers to set up real-time PCR (qPCR) experiments. 

LISTED UNDER: QPCR

DNA Polymerase Offers Specificity, Stability for PCR

March 17, 2015 1:03 pm | Product Releases | Comments

The FlashTaq Hot-Start Taq DNA Polymerase from Empirical Bioscience is a chemically-modified Taq polymerase designed to reduce non-specific amplification in PCR.

LISTED UNDER: DNA Polymerases

Sequencing-ready Library Construction

March 6, 2015 1:55 pm | Product Releases | Comments

The NEXTflex Rapid DNA-Seq Kit from Bioo Scientific enables construction of sequencing-ready libraries in less than two hours, with a flexible input range from 1 ng to 1 µg of DNA. A bead-based, gel-free size selection protocol and the availability of up to 96 unique adapter barcodes facilitate high-throughput applications.

LISTED UNDER: Library Construction

Automated Next Generation Sequencing Applications

March 4, 2015 4:30 pm | Product Releases | Comments

Beckman Coulter Life Sciences, through a partnership with Illumina, offers automated methods to improve processes and throughput in next generation sequencing (NGS) sample preparation. Under the agreement, Beckman Coulter will use its experience in automated NGS sample preparation to develop, distribute and support automation for Illumina’s TruSeq and Nextera sample preparation kits and Illumina will provide technical expertise on chemistry and protocols.

LISTED UNDER: Automated Liquid Handling Systems | Automated Sequencers

Single-base Resolution Sequencing Kits

March 3, 2015 4:13 pm | Product Releases | Comments

Cambridge Epigenetix launched TrueMethyl kits. TrueMethyl provides quantitative, accurate and repeatable single-base resolution sequencing of the modified bases hydroxymethylcytosine (5-hmC) and methylcytosine (5-mC).

LISTED UNDER: Sequencing

ddPCR Kit for Next-gen Sequencing

March 2, 2015 10:04 am | Product Releases | Comments

Bio-Rad Laboratories offers a ddPCR library quantification kit for Illumina TruSeq sample preparation protocols. Used with Bio-Rad's QX100 Droplet Digital PCR system, the kit offers researchers a way to precisely and directly measure amplifiable library concentrations.

LISTED UNDER: PCR Product Quantitation

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