White Papers
Evaluation of a New Nano-Type UV-Vis Spectrophotometer
March 3, 2011 4:11 amAnalysis of one- to four-microliter size samples for nucleic acids has become routine in many life science laboratories. However, until now, available instruments require considerable manipulation of the instrument and sample; some require manually recording the data. The user must typically lower and raise the arm manually, then wipe the sample manually from the target after each analysis. And fiberoptics used in some of these instruments are subject to deterioration.
Production of Recombinant Proteins and Monoclonal Antibodies in Hollow Fiber Bioreactors
January 25, 2011 10:09 amWhile well-understood, robust and convenient, classical batch-style 2-D culture on non-porous supports or 3-D suspension culture in other devices are really not very biologically relevant models. Cell culture conditions can affect the quality of the antibody or protein produced.
Selecting Robots for Use in Drug Discovery and Testing
December 6, 2010 8:07 amDrug discovery and testing, with their need for speed, repeatability and verification, are ideally suited to benefit from robot automation. It is therefore not surprising that robots have been at the forefront of automation developments in both these areas.
HP Scalable Network Storage Systems for Life Sciences
September 13, 2010 7:00 amLife sciences research today is advancing exponentially, each step bringing us closer to the realization of truly personalized medicine–preventive care and treatments designed specifically for each individual. In the near future, PCPGM healthcare researchers expect to be able to use predictive genetic testing to create custom treatment plans for individuals and deliver dramatic improvements over today’s one-size-fits-all approach. But research capabilities are only part of the equation; current storage and operating capacities must also evolve to accommodate ever-expanding amounts of data before the goal of personalized medicine can be realized.
Step up to the MIQE
March 30, 2010 9:59 am | by Richard Kurtz, PhDOver the years, polymerase chain reaction (PCR) has evolved into a readily automated, high throughput quantitative technology. Real-time quantitative PCR (qPCR) has become the industry standard for the detection and quantification of nucleic acids for multiple application, including quantification of RNA levels. But a lack of consensus among researchers on how to best perform and interpret qPCR experiments presents a major hurdle for advancement of the technology. This problem is exacerbated by insufficient experimental detail in published work, which impedes the ability of others to accurately evaluate or replicate reported results.
Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient
March 30, 2010 9:09 amThe year 2009 was marked by the emergence of a novel influenza A (H1N1) virus that infects humans. There is a need to identify the different strains of influenza virus for purposes of monitoring the H1N1 strain pandemic and for other epidemiological and scientific purposes.
Advantages of Monolithic Laser Combiner Technology in Confocal Microscopy Systems
January 6, 2010 8:18 amFluorescence microscopy techniques require a reliable light source at the desired wavelength or wavelengths, with minimal downtime for maintenance and alignment. Lasers are a popular light source, although the alignment and upkeep of laser combiners is a time-consuming prospect for many users.
Size-Exclusion Chromatography for Purification of Biomolecules
December 2, 2009 9:21 amSize-exclusion chromatography (SEC) is a popular method to separate biomolecules based on their size. Primarily, it is applied to the separation of biopolymers such as proteins and nucleic acids, i.e. water-soluble polymers.
Improving Separation During Electrophoresis
December 2, 2009 9:10 amSeparateIT gels represent a novel gel matrix for DNA electrophoresis. Gel polymers are arranged in a conceptually different way, in accordance with a new theoretical model of gel electrophoresis.
Improving Quality of ELISA
December 2, 2009 9:00 amUsing ready-to-use ELISA kits from manufacturers is easy and convenient. Sometimes however, home-made ELISA is required because there is no kit available with the right antibodies or the characteristics of the available kits such as their limits of detection are not appropriate.
Nucleic Acid Decontamination with the ExitusPlus Technology
December 2, 2009 6:54 amUsing new methods that detect extremely low levels of DNA molecules, researchers investigated the molecular mechanism of action of various commercially available DNA decontamination reagents. They found that when using high concentrations of DNA and short incubation times, none of the conventional reagents destroyed DNA molecules efficiently, despite their corrosive or even toxic properties.
The Future of the FDA: Operating in an Electronic World
October 12, 2009 2:04 pm | by AxendiaAcording to an Axendia reseach study, the FDA is currently shifting its organizational and technology infrastructures to facilitate electronic interactions with the companies it regulates.
Tissue Microarrays - Opening up new opportunities in the Therapeutics sector
October 12, 2009 2:01 pm | by OcimumTissue Microarray has become a pivotal cog in the wheel for high throughput studies and is an integral part in the validation process for screening results from discovery platforms for expression studies using various approaches.
De Novo Formula Generation with Sub-ppm Confidence
October 12, 2009 1:40 pm | by Bruker DaltonicsNovel technology embodied in the micrOTOF allows precise measurement of both accurate mass and True Isotopic Pattern (TIP) over a wide dynamic range, allowing for the implementation of an open access system.
PLA 2.0 Software for Analyzing - Parallel-Line and Parallel-Logistic Assays
October 12, 2009 1:27 pm | by STegmann SystemsBiological or potency assays are frequently analyzed with the help of the parallel-line or parallel-logistic (4 or 5 parameter fit) methods. These methods have major advantages over traditional single-point assays.
