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Improving Quality of ELISA


Using ready-to-use ELISA kits from manufacturers is easy and convenient. Sometimes however, home-made ELISA is required because there is no kit available with the right antibodies or the characteristics of the available kits such as their limits of detection are not appropriate. Ready-to-use ELISA kits from good suppliers may be stored for two years at 4°C without any problem. With home-made ELISA it is a completely different story. For any new measurement one has to coat a new plate, because after storage of some days the plates don’t perform as well as before.

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