Cultivation of a PER.C6 EpCAM Cell line in CDM4PERMab Powder Medium Reconstituted with Arium pro UF WaterJanuary 23, 2013 2:33 pm | by Anil Kumar Rathod, Sheokant Diwakar & Dr. Ashok Mundrigi, Sartorius Stedim India; Dr. Elmar Herbig, Sartorius | Articles | Comments
Water is a major component of all cell culture media and is therefore needed to prepare media, buffers, and additives, and as well as to serve many ancillary functions, such as heating, cooling, cleaning and rinsing. Thus, water quality plays an important role in the outcome of cell culture experiments.
The new arium pro from Sartorius Stedim Biotech produces ultrapure water, or also known and Type I water according to ASTM standards. The water produced is void of all charged contaminants and removes other contaminants such as heavy metals and organics.
PURELAB water purification systems feature multi-stage monitoring and purification technology that reduces annual costs. A complete system sanitization function reduces risk of bacterial contamination. Real-time TOC monitoring is offered on some models.
The RiOs-DI Clinical water purification system is optimally used with low-volume analyzers requiring between 10 - 30 L purified water per day. It provides a reliable alternative to buying, storing and handling bottled water.
The Simplicity system produces ultrapure water on demand from pretreated water (Elix system distilled, deionized or reverse osmosis water).
Highly purified water has become a standard tool in molecular biology. Techniques such as reverse osmosis, electrodeionization, ultrafiltration, and chemical treatments provide water of varying quality and utility. Water quality specifications differ for applications such as liquid chromatography, in situ hybridizations, single nucleotide polymorphism analysis, and DNA amplification.
Millipore has announced a complete line of consumables and accessories for its Milli-Q water purification systems. Plug-in Q-Gard® purification packs are tailored to particular feed water sources.
Milli-Q® Biocel ultrapure water system is offered for applications such as affinity chromatography, protein and nucleic acid isolation, ELISA tests, electrophoresis gel make-up and serum-based cell and tissue culture media preparation.