PromoFluor dyes span the wavelength spectrum from blue-green to far red. They provide photostability, strong absorption, high fluorescence quantum yield and water solubility. Dye intensity increases when coupled to biomolecules resulting in reduced influence of uncoupled dyes.
Imaging systems used for Western blot analysis are often described as 'quantitative'. While it is true that the instrumentation used to image the blots does acquire signal in a quantitative way, data produced by these systems can only be quantitative if the signal generated by the chemistry is proportional to the amount of sample on the blot in a linear fashion.
The clock ticks forward one second. A technician pipets a sample from a crude cellular extract, processes it through a complex preparation protocol with multiple reagents, runs the processed extract on a gel, isolates the desired bands, and characterizes the activities of several novel enzymes.
Proteomics-grade trypsin is prepared in a 15 minute digestion using the Trypsin Spin Column, Proteomics Grade. The ultra-micro spin column contains purified, TPCK-treated porcine trypsin immobilized on a spherical 20 micron silica support, chemically modified to minimize non-specific adsorption.
Somewhere, deep within a complex cell extract sample, sit the potential biomarker proteins Dr. Pierre Thibault is hoping to profile and characterize. The barrier to finding them? Everything else in the sample.
Proteus software now includes Automated Informatics, a technology framework that enables bioinformatics pipelining and analytics automation.
AXIMA-TOF 2 MALDI TOF-TOF mass spectrometer, a high-energy, collision induced dissociation (CID) MS/MS instrument, extends the type of analyses life science laboratories can provide. Intuitive software allows for use in open-access lab environments, requiring minimum user input.
CLINPROT FORUM supports customers in protein/peptide biomarker discovery and validation, as well as in clinical and applied proteomics.
Version 4.0 of PathwayStudio (formerly PathwayAssist, Ariadne Genomics, Inc.) contains new tools for microarray data analysis and pathway dynamics simulation, as well as over 1000 reconstructed pathways.
Integrated proteomics product line is offered for producing, imaging and analyzing 2D gels. IEF-SYS, a one-tank electrophoresis system, allows 2D gels to be run with both immobilized pH gradient strips and pre-cast acrylamide gels.
ProLink Express automated proteomics work-cell integrates all plate delivery and transfer operations with automated data tracking, from initial bacterial colony picking through final protein expression and measurement. The system links all the components needed for DNA expression of gene libraries.
Proteomics, the successor to genomics, has rapidly assumed a central role in modern biology and medicine. Where the human genome contains about 22,000 coding genes, the functional proteome is comprised of anywhere from fifteen to a hundred times as many proteins.
The Agilent 6340 Ion Trap Liquid Chromatograph/Mass Spectrometer (LC/MS) fits on a lab bench and is equipped with an electron transfer dissociation (ETD) unit a new tool for the study of proteins and post-translational modifications.
LTQ Orbitrap hybrid mass spectrometer is offered for small molecule research, drug discovery, proteomics, metabolite identification, and metabolomics.
Listing of awarded and available funding
Background fluorescent emission of Immobilon-FL (blue) to nitrocellulose and PVDF at commonly used excitation wavelengths.
Listing of collaborations
Source: Arizona State University, Tempe
Listing of awarded and available funding
His GraviTrap 1 ml pre-packed columns purify proteins for use in functional and structural genomics and proteomics research. The columns purify histidine-tagged proteins from clarified or unclarified samples without the need for a pump or purification system. Protein binding capacity is approx.
Sarah Brautigam, Coordinator, NIH Neuroscience Consortium, prepares an array used in an autism study with the Affymetrix GeneChip Fluidics station at TGen. By Angelo DePalma Within a few years of their introduction, microarrays have become a mainstay of biological research.
Dr. K.W. Michael Siu, Professor of Chemistry and NSERC/MDS SCIEX Industrial Research Chair, York University, Toronto, Canada. By Mark Springer A group of Canadian researchers led by Dr. K.W.
With the emergence of RNA interference (RNAi) as a powerful analytical tool in mammalian genetic screening, the RNA research field has seen a marked resurgence in its vitality.
PathwayExpert is an enterprise class software platform for pathway analysis and systems biology, and allows researchers to analyze microarray and proteomics data, to create and share custom pathways for diseases, drug targets, biomarkers, and to perform literature mining.
SeraFILE 10 is a subfractionation methodology for differential proteome analysis. Electrophoretic profiles of serum subfractions demonstrate good resolution and quantification. Carryover from the three highest abundance serum proteins, albumin, IgG and transferrin, is minimal.