Prespotted AnchorChip, a second-generation disposable MALDI target for gel-based and LC-MALDI-based expression proteomics, eliminates cross-contamination from previous samples and calibration spot preparation and reduces sample preparation time.
Charles River Proteomic Services Inc. (Worcester, MA) and GenWay Biotech Inc. (San Diego, CA) have collaborated to produce avian IgY polyclonal antibodies that function as a fractionation step prior to protein separation.
The ultraflex II MALDI-TOF and TOF/TOF mass spectrometer (Bruker Daltonics) finds application in peptide de novo sequencing, the analysis of post-translational modifications, and top-down protein primary structure determination.
Genome of Deadly Pathogen Sequenced
Computationally spotting clusters from cell images
Sharing Designs for custom microarrays
A glimpse of what's ahead in medicine
Microarray technology has come a long way since Affymax, predecessor to Affymetrix (Santa Clara, CA), introduced polypeptide arrays in the late 1980s. Today's gene, protein, and tissue arrays have evolved from research curiosities to the experimental platform of choice for many life scientists and clinical researchers - something akin to high-tech, ultraminiaturized microtiter plates.
MagAB nanoparticle allows scientists to attach antibodies, antibody fragments, peptides, DNA or ligands to magnetic bead surfaces. The beads are valuable to researchers looking for specific proteins or binding partners, and for biologically selective and very sensitive specific clinical assays.
MFP fluorescent dyes are ready for coupling to proteins and nucleic acids, and span the wavelength spectrum from green to far red. They offer photostability, a strong absorption, high fluorescence quantum yield and good water solubility.
Dymension software accurately analyzes 2D gels in seconds. A spot finding algorithm results in minimal post editing being needed and couples with an automatic gel alignment process to speed the whole imaging process right through to results and report generation.
Silicon Graphics Prism Linux OS-based computers combine standards-based Intel Itanium 2 processors, the Linux operating environment, and advanced graphics technology to addressing demanding visual computing problems.
LS*LIMS Software from Applied Biosystems increases lab productivity by integrating Applied Biosystems and third-party instrument interfaces, including the Applied Biosystems 3730 and 3730xl DNA Analyzers, the ABI PRISM 7900HT Sequence Detection System, and the GeneAmp PCR System 9700.
The Proteineer-LC is an LC-ESI-MALDI system for LC-based expression proteomics workflows. Its manufacturer, Bruker Daltonics, also offers Top-Down Proteineer capability on the apex-Qe Q-q-FTMS mass spectrometry system.
NCI Supports Biomarker Discovery
Stepping up biomarker discovery
Integrated gene expression and genotyping technologies
Polymer Laboratories has launched a range of capillary and micro chromatography columns for small-scale chromatography. Chemically stable polymeric particles do not leach either soluble or particulate species.
By Elizabeth Tolchin
Instrumentation, software, columns, and method protocols are integrated in this complete system for identification of proteins and biomarkers. A manual of optimized method protocols is included for each step of the MS-based proteomics workflow, from sample preparation to data analysis and reporting.
Sample preparation in biological and biotechnological systems plays an important role in the success of many processes. It is a requirement that the material of the micro test tubes used must possess low affinity for proteins and peptides to prevent adsorption of the sample material to the tube surface.
MDLC LTQ LC/MS system (Thermo Electron Corp.) provides experimental flexibility and precision for proteomics solutions.
By Catherine Shaffer - 8/03/04
Serum Biomarker Chips facilitate profiling of serum biomarkers and discrimination of stages of disease progression. Detection of over- and under-abundant serum biomarkers has value in cancer research. Proteomics researchers can profile and pattern the molecular signature of human serum.