AMSBIO launched a new, highly purified Chondroitinase ABC. This new enzyme is supplied protease-free, carrier-free and with low-endotoxin levels for use in neuroscience and regenerative medicine research.
Antibodies available for the pre-clinical drug development marketplace identify key enzymes and transcription factors important in drug metabolism and its regulation.
The Sigma-Aldrich Corp. Assay Library features over 600 detailed procedures for measuring enzyme activity and related metabolites. It is available on the company's Enzyme Explorer, which also features in-depth resources on protein research, including metabolic pathway animations.
Enzymes, biochemicals and primary cell isolation kits are offered for application in life science research, diagnostics and biotechnology. New and traditional products in the line find application in cell culture, protein research, diagnostic enzymology and reagent production, and molecular biology.
Recombinant mouse Tryptase-5/Prss32 is encoded by the Prss32 gene. The mT5/Prss32 is a member of the mouse serine protease superfamily and is composed of a signal peptide, a pro region, a catalytic domain and a C-terminal hydrophobic peptide.
PTEN (phosphatase and tensin homolog) activity assay is offered in a non-radioactive 96-well ELISA format that directly measures PTEN activity in vitro , and that specifically measures the lipid product of PTEN.
MonsterScript Reverse Transcriptase is a highly processive thermostable enzyme that completely lacks RNase H activity. The enzyme is fully active up to 65 C, which enables reverse transcription through regions of difficult secondary structure.
Cayman Chemical Renin Inhibitor Screening Assay Kit is offered in a 96-well format for evaluating human renin inhibitors. The assay utilizes a renin-based synthetic peptide substrate which incorporates the fluorophore EDANS at one end and an EDAN-quenching molecule (Dabcyl) at the other end.
PDELight HTS cAMP Phosphodiesterase Assay Kit screens for phosphodiesterases in high throughput applications. The homogeneous assay uses bioluminescent detection. Phosphodiesterases are enzymes in the cell that act as gating mechanisms.
Ras GTPase Chemi ELISA detects and quantifies activated Ras. This ELISA-based kit is sensitive, and its high throughput format enables simultaneous screening of 1 to 96 samples in a single experiment.
BacLite RapiTECT GN , based on Adenylate Kinase (AK) technology, is offered for the enumeration of gram-negative bacterial cells. Typical enumeration results can be achieved in minutes, and growth-based results within a few hours.
An In Vitro Assay for the Quantitation of PARP Activity in Cell Extracts and for Determining the Efficacy of PARP InhibitorsJuly 13, 2005 8:28 am | Articles | Comments
By Leonard S. Bazar, PhD click the image to enlarge Figure 1. Colorimetric readout of PARP activity. Panel A illustrates the blue color and Panel B the yellow color that results from the addition of acid.
APex Heat-Labile Alkaline Phosphatase uses a single, convenient protocol to dephosphorylate blunt, 5 ft.-overhang and 5 ft.-recessed DNA ends, regardless of the length of the overhang.
Recombinant human Kallikrein 4 antibody, now available from R&D Systems, is also known as Prostase and Enamel Matrix Serine Protease 1 (EMSPI). KLK4 is a recently characterized member of the Kallikrein family of serine proteases.