ddPCR Kit for Next-gen Sequencing
Tue, 04/29/2014 - 1:12pm
Bio-Rad Laboratories offers a ddPCR library quantification kit for Illumina TruSeq sample preparation protocols. Used with Bio-Rad's QX100 Droplet Digital PCR system, the kit offers researchers a way to precisely and directly measure amplifiable library concentrations. The TruSeq sample preparation method is the technique behind Illumina's MiSeq and HiSeq next-generation sequencing (NGS) platforms. Using the kit to quantify TruSeq DNA libraries maximizes the number of useable reads, enables consistent loading and optimizes the utilization of every sequencing run. The resulting data provide additional measures of library quality not provided by other methods, including the percentage of nonamplifiable species such as adaptor dimers as well as the size range of library inserts. The kit also reduces PCR bias due to sample partitioning during quantification, easily incorporates into the TruSeq library construction workflow and provides fluorescence amplitude data, a metric for library quality. Kits for other NGS platforms are also in development.
Bio-Rad Laboratories Inc., 800-424-6723, www.bio-rad.com