Bisulfite Conversion System for DNA Methylation Analysis
Promega has launched the MethylEdge Bisulfite Conversion System—a system offering an efficient method for rapidly performing bisulfite conversion and DNA clean-up within two hours, while maintaining DNA integrity to provide more intact, converted DNA for a variety of sensitive applications. MethylEdge requires only small amounts of input genomic DNA (100pg-2µg). Using optimized conversion reagents, clean-up and recovery methods, the system converts DNA at ≥90% efficiency with minimal fragmentation. The resulting DNA is suitable for use in downstream applications such as PCR, array, or sequencing assays. The kit includes all reagents necessary to perform 50 bisulfite conversion reactions. All MethylEdge reagents can be stored at room temperature and require minimal up-front preparation. Control DNAs are also available separately and can be used as standards in both the conversion reaction and post-conversion analysis.
Promega Corp., 608-274-4330, www.promega.com/methylation