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Nucleic Acid Size Selection for Fragment Library Prep

Wed, 12/05/2012 - 11:37am

Beckman Coulter Life Sciences SPRIselectSPRIselect from Beckman Coulter Life Sciences utilizes SPRI (Solid Phase Reversible Immobilization)-based chemistry to speed and simplify genomic DNA size selection for next generation sequencing fragment library preparation. Following shearing, the library construction process requires size selection to produce uniform distribution of fragments. SPRIselect allows size distribution to be adjusted between 150 and 800 base pairs to suit the application and sequencing platform. The process can be performed manually or automated for high throughput in 96-well plates on platforms such as Beckman Coulter Life Sciences’ Biomek Workstations. SPRIselect reagent kits are available in 5, 60 and 450 mL volumes, enable rapid and consistent size selection, and come with guidelines to assist users in customizing protocols. Gel cartridges, chips and additional instruments are not needed. Samples should be fragmented, double-stranded DNA of 50 ?L or greater, dissolved in molecular biology grade water or such standard buffer solutions as Tris or TE. SPRIselect is ideal for use in most common next generation applications, including paired-end, single-end, targeted, ChiP and RNA sequencing, and for use with samples to be run on such platforms as Illumina’s MiSeq, HiSeq and Genome Analyzer IIx; Roche’s 454 GS FLX+ and GS Junior; and Life Technologies’ SOLiD series and Ion Torrent PGM. Beckman Coulter, Inc., 800- 742-2345, www.spriselect.com

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