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Reproducible Gene Delivery for Stem Cell Research

Thu, 08/05/2010 - 12:03pm

AMSBIO lentivirus supernatant productsAMSBIO have announced a range of ready-to-use lentivirus supernatant products suitable for many kinds of gene delivery applications including stem cell research. Converting fully differentiated mouse or human somatic cells into embryonic-like cells (so called induced Pluripotent Stem Cell: iPSC) has attracted enormous attention in stem cell research. Multiple reports have demonstrated that iPS cells were generated by using a set of transcription factors or stem cell factors that delivered as expression virus or expressed proteins. Although the combination of reprogramming factors may slightly different, the main stem cell factors are: OCT3/4, SOX2, NANOG, LIN28, c-Myc and KLF4. iPSC holds the promise of curing many human diseases and accelerates the stem cell research. Using the SureTiter lentiviral system from AMSBIO, users can generate high-titer lentiviral particles for all six human stem cell factors. Each factor was fully sequencing verified and matched to the CDs in NCBI database. High titer lentiviral particles / supernatant were produced in 293T packaging cells in DMEM with 10% heat-inactivated FBS. They are psudotyped with VSV-G glycoprotein-attached vector map. Prepared using proprietary protocols to integrate a real-time fluorescence monitoring tag in the system - each 200?l vial of AMSBIO lentivirus supernatent contains a high titre of highly transducible lentivirus (1 x 10 e7 IFU/ml). Each vial of lentiviral supernatent particles contains a fully sequence verified target, ready for transduction into any mammalian cells. AMS Biotechnology (AMSBIO), +44-1235-828200, www.amsbio.com/custom-lentivirus-service.aspx

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