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qPCR Data Analysis Software

Tue, 03/30/2010 - 9:46am

Bio-Rad Biogazelle qbasePLUSBio-Rad Laboratories announced the exclusive availability of Biogazelle’s qbasePLUS, qPCR data-analysis software for Bio-Rad’s CFX96 and CFX384 real-time PCR detection systems. qbasePLUS helps to provide researchers with accurate gene expression analysis of their real-time PCR data by using proven solutions for data quality control, including the elimination of erroneous data, normalization for removing sample-specific non-biologic variation, and inter-run calibration, which can remove the technical variation between samples analyzed in different runs. Enhancing the reliability of the qPCR data, qbasePLUS conforms to the emerging qPCR best practices standard: Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE), which defines the minimum information required for the evaluation of qPCR results. Using the CFX96 and CFX384 with qbasePLUS software saves time and accelerates research through automated, fast calculations and direct import of CFX96 and CFX384 data into qbasePLUS. The combined solution provides researchers with the flexibility to handle both small and large experiments as well as combine data from different experiments using qbasePLUS software as a repository database.

Using the Bio-Rad C1000 thermal cycler chassis as the base thermal cycler instrument, the CFX96 and CFX384 real-time PCR detection systems incorporate optical technologies and software to deliver sensitive, reliable detection for real-time PCR applications, including absolute quantitation, genetic variation analysis, and gene expression. Both systems read each well individually with high sensitivity and no cross talk to deliver optimal quantitative results. The CFX96 and CFX384 systems provide excellent performance in uniformity, dynamic range of detection and multiplexing capabilities, and they can be operated in several control configurations, including a stand-alone mode with no computer attached. With the CFX384 system, researchers have access to high-throughput real-time PCR in a 384-well format without having to sacrifice convenience and flexibility. Bio-Rad Laboratories, Inc., 800-424-6723, www.bio-rad.com/ amplification/

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