Multiplex Cytokine, Chemokine ELISAs
Tue, 10/16/2007 - 11:04am
Quansys Multiplexed ELISA Human Cytokine - IR (9-plex), Human Chemokine - IR (9-plex), Mouse Cytokine - IR (9-plex), and Mouse Chemokine - IR (9-plex) assays are central to kinase drug discovery programs for indications including oncology, inflammation, metabolic, cardiovascular and neurological diseases. These arrays utilize a pre-spotted plate with nine different capture antibodies per well. Following a simple ELISA procedure, infrared signal is captured with an Odyssey or Aerius infrared imaging system (LI-COR Biosciences, Lincoln, NE). The image is analyzed using array software and the intensity of the spots for each unknown sample is compared with standard curves and exact values of each biomarker (pg/ml) are calculated.