Realtime Stain from Biophoretics is a single step coomassie like pre-electrophoresis protein stain that allows visualizing the separation as it occurs; in real time. The stain irreversibly binds amine groups coloring the proteins deep blue with little to no gel background; providing detection sensitivity as low as 50ng. Seeing the separation as it occurs provides knowledge that the experiment is proceeding as planned.
Realtime stain is specially formulated for use in SDS polyacrylamide gel electrophoresis (SDS-PAGE). It is optimized as a simple single step protocol for pure and partially-pure protein samples, markers, recombinant protein fragments, FPLC fractions and antibodies. It comes as 2× concentrate sufficient for 400 samples.
Biophoretics, Inc., 800-691-6461, www.biophoretics.com