MagSi-DNA cleanFIX from AMSBIO offers a solution for purifying DNA fragments, removing all unwanted side products and reagents, such as single nucleotides, terminator dyes, primers and primer dimers, enzymes, buffers, and salts. In many genomics applications, purification of DNA fragments is required to prevent downstream interference with analysis methods. Purification of PCR reactions often requires removal of dNTPs, primers, enzymes, salt by-products and various additives. Historically purification protocols have involved different sample types, different volumes and often multiple washing and centrifugation steps. Using such methods the errors incurred during sample preparation have resulted in lost time and significant costs due to re-work and lost reagents.
MagSi-DNA cleanFIX enables a combination of PCR clean-up and dye terminator removal from sequence reaction mixes with a single product. The kit uses magnetic silica bead technology, and is easily automated because no columns or centrifugation steps are involved. The kit uses a simple “Bind, Wash & Elute” procedure common to magnetic particle purification protocols.
Independent test results demonstrate how MagSi-DNA cleanFIX purification methods developed for a commercial liquid handling workstation provide an automated walk-away solution for PCR clean-up and dye terminator removal from sequence reactions. The precision and reproducibility of the workstation in combination with the customized magnets for MagSi-DNA cleanFIX are shown to allow fast magnetic separation and homogenization of the samples. Overall the PCR clean-up protocol using MagSi-DNA cleanFIX is shown to enable fast and efficient recovery of DNA fragments larger than 80 bp with >99% removal of primers and primer dimers. In addition the dye terminator removal method consistently delivered high quality sequence data with Phred >20 scores above 700.
AMSBIO LLC, 800-987-0985, www.amsbio.com