This new guide from Hugo-Sachs Elektronik-Harvard Apparatus GmbH, includes systems for all model species (mouse through pig), full respiratory mechanics in one system, new pig lung system for translational research and modular systems that allow custom configuration. The Isolated Perfused Lung (IPL) technique has been found to be invaluable in characterizing the non-respiratory capabilities of pulmonary tissues such as pulmonary metabolic activity as well as the activities of various components (pulmonary alveolar macrophage, alveolar tissue, endothelial tissue, etcetera) in response to inhaled/airborne particulates/therapies. Isolated lung systems are equally useful for evaluating respiratory functions such as respiratory mechanics and gas exchange. The lungs, while being removed from the body, are still an intact functional organ, unlike other in vitro methods such as slices and cultures, and therefore can continue in their physiological function without the interference from the CNS and other systems of the living organism. Building your own isolated lung system is a challenging endeavor that requires time and resources that are in an increasingly short supply. This new guide explains the requirements of an isolated lung system, includes references, and provides systems that are largely configured out of the box– simply reconnect tubing and transducers. The design of the IPL series of lung systems allows for a higher reproducibility and lower inter-user variability than traditional tubing/glassware systems.
Harvard Apparatus, 800-272-2775, www.harvardapparatus.com