Molecular Devices announced the launch of FLIPR Calcium 6 Assay Kits, the latest addition to their range of calcium assay kits to address diversified GPCR and ion channel targets. Featuring a proprietary fluorophore, the dye offers a highest quantum yield, delivering the greatest signal window available in a calcium assay kit. This substantial increase in signal enables researchers to monitor low-responders, including biorelevant reactions from endogenous, primary or stem cell targets. The kits provide a method for detecting intracellular calcium changes in a simple and reliable homogeneous assay format. Combining the novel fluorophore with proven proprietary quench technology, the new assay affords the highest signal to background ratio. The dye is more resistant to anion-exchange proteins, enabling measurement with minimal to no probenecid, an exchange inhibitor, when studying cell lines containing an organic anion transporter. Using a quench-free protocol, the kit allows assay performance without a masking technology, making it an ideal choice for quench-sensitive applications such as targets sensitive to quenchers or multiplexing with luminescent indicators. The new kits support GPCR targets that can be run on Molecular Devices’ FLIPR Tetra and FlexStation 3 Instrumentation.
Molecular Devices, www.moleculardevices.com/FLIPR_Calcium6