XPF /ERCC1 forms a heterodimer complex and catalyzes 5’ incision to the DNA damage during nucleotide excision repair (NER). ERCC1/XPF is also required for repair of DNA interstrand crosslinks and involved in repair of double strand breaks. Mutations in ERCC1 or XPF cause xeroderma pigmentosum, XFE progeroid syndrome or cerebro-oculo-facio-skeletal syndrome, characterized by increased risk of cancer, accelerated aging and severe developmental abnormalities, respectively. High ERCC1-XPF expression levels have been correlated with the resistance to chemotherapy and provide as a potential biomarker to estimate DNA repair nuclease activity. Trevigen’s Monoclonal XPF Antibody (4422-AMC-100) recognizes human XPF and is suitable for Western blotting and immunocytochemistry. It is a purified mouse monoclonal antibody raised against full length recombinant human XPF/ERCC1 complex.
Trevigen, Inc., 800-873-8443, www.trevigen.com