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Audio presentation of interviews, roundtables, and more.

Futures in Biotech 47: Genetic Engineering in the 21st Century

Dr. Oliver Smithies discusses the present and future of genetic engineering.

Dr. Barbara Karn - Nanotechnologies and Environmental Concern - 2.08.08

February 8th, 2008 - Nanotechnologies and Environmental Concern Speaker: Dr. Barbara Karn U.S. Environmental Protection Agency - National Center for Environmental Research, Head of the Research Grant Program for Nanotechnologies in Office of Research and Development, Member of Project on...

Dr. Nigel M. de S. Cameron - Nanotechnologies and Medical Ethics - 3.03.08

March 3rd, 2008 - Nanotechnologies and Medical Ethics Speaker: Dr. Nigel M. de S. Cameron President of the Institute on Biotechnology and the Human Future, Director of the Center on Nanotechnology and Society, Research Professor of Bioethics and Associate Dean at Chicago-Kent College...

Dr. David Resnik - Conflicts of Interest in Biomedicine - 4.02.08

April 2nd, 2008 - Conflicts of Interest in Biomedicine Speaker: Dr. David Resnik Bioethicist, National Institute of Environmental Health Services and National Institutes of Health

Dr. Eric Meslin - Ethical Issues in Constructing and Using Biobanks - 4.16.08

April 16th, 2008 - Ethical Issues in Constructing and Using Biobanks Speaker:Dr. Eric Meslin Director of the Indiana University Center for Bioethics, Professor of Medicine, and of Medical and Molecular Genetics in the Indiana University School of Medicine, Professor of Philosophy in the...

Dr. Bernard Rollin - The Use of Animals for Research - 11.4.08

November 4th, 2008 - Topic: The Use of Animals for Research Speaker: Dr. Bernie Rollin, University Distinguished Professor, Professor of Philosophy, Biomedical Sciences, and Animal Sciences, University Bioethicist at Colorado State University

Dr. Leigh Raymond - The Ethics and Politics of Climate Change - 11.05.07

November 5th, 2007 - Ethics and Politics of Climate Change Speaker: Dr. Leigh Raymond Associate Professor of Political Science, Associate Director for the Purdue Climate Change Research Center

Dr. Samuel A. Richmond - Who Owns Truth? Biomedical Research in a Health Policy Context - 2.19.2009

Dr. Samuel A. Richmond Professor of Philosophy and Co-director Bioethics Center, Cleveland State University Who Owns Truth? Biomedical Research in a Health Policy Context February 19, 2009

Futures in Biotech 56: New Antiviral Strategies With Karla Kirkegaard

Hosts: Marc Pelletier and Vincent Racaniello A look into RNA viruses and more. Guest: Dr. Karla Kirkegaard, professor and chair of the Department of Microbiology and Immunology at the Stanford University School of Medicine Show notes Comments and suggestions on Futures in Biotech. For a...

Futures in Biotech 57: Mechanisms Of Non-Mendelian Inheritance In Evolution

Hosts: Marc Pelletier and George W. Farr, Ph.D., vice president of biochemistry and biophysics at Aeromics and adjunct professor of physiology and biophysics at Case Western Reserve University How prion proteins can act in non-mendelian inheritance, or evolution without DNA. Guests:...

Futures in Biotech 58: Vertical Farms and much more with Dick Despommier

Hosts: Marc Pelletier and Vincent Racaniello, Ph.D., Professor of Microbiology & Host of This Week in Virology, Host of This Week in Parasitism, Columbia University, New York, NY. Feeding the world with indoor vertical farming. Guest: Dickson D. Despommier, Ph.D., Professor of...

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Research Exchange

Microplate Reader Test Plates Ensure Reliable Microplate Reader Operation

Aug 20

Use of a certified test plate can help to increase efficiency when calibrating microplate readers.

Automated Imaging Assisted Cell Line Development

Aug 19

Improve titer, quality and speed in cell line development.

New Frontiers: The Changing World of Biospecimen Collection and Management

Jul 30

The growth of biomarker research and personalized medicine requires more unified and large-scale software systems to track biological materials.

Optical Imaging in Stem Cell Research

Jun 1

Developing sensitive, non-invasive technologies to monitor engraftment in vivo is essential to accelerate the clinical implementation of cell therapies.

Step up to the MIQE

Mar 30

Over the years, polymerase chain reaction (PCR) has evolved into a readily automated, high throughput quantitative technology. Real-time quantitative PCR (qPCR) has become the industry standard for the detection and quantification of nucleic acids for multiple application, including quantification of RNA levels. But a lack of consensus among researchers on how to best perform and interpret qPCR experiments presents a major hurdle for advancement of the technology. This problem is exacerbated by insufficient experimental detail in published work, which impedes the ability of others to accurately evaluate or replicate reported results.

Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient

Mar 30

The year 2009 was marked by the emergence of a novel influenza A (H1N1) virus that infects humans. There is a need to identify the different strains of influenza virus for purposes of monitoring the H1N1 strain pandemic and for other epidemiological and scientific purposes.

Advantages of Monolithic Laser Combiner Technology in Confocal Microscopy Systems

Jan 6

Fluorescence microscopy techniques require a reliable light source at the desired wavelength or wavelengths, with minimal downtime for maintenance and alignment. Lasers are a popular light source, although the alignment and upkeep of laser combiners is a time-consuming prospect for many users.

Size-Exclusion Chromatography for Purification of Biomolecules

Dec 2 2009

Size-exclusion chromatography (SEC) is a popular method to separate biomolecules based on their size. Primarily, it is applied to the separation of biopolymers such as proteins and nucleic acids, i.e. water-soluble polymers.

Using the Tecan Genesis Workstation to Automate a Cytometric Bead Array (CBA) Immunoassay

Mar 11

The poster describe the process involved in automating a Cytometric Bead Array (CBA) immunoassay developed to measure relative concentrations of serum antibodies against Tetanus (TT), Sperm Whale Myoglobin (SWM) and Keyhole Limpet Hemocyanin (KLH) in KLH-immunized volunteers.

Ensuring Quality in Assays Performed with Automated Liquid Handlers

Feb 2

The focus of this presentation is to highlight the need of ensuring quality in important assays performed with automated liquid handlers. Nearly all assays performed within a laboratory are volume-dependent. In turn, all concentrations of biological and chemical components in these assays, as well as the associated dilution protocols, are volume-dependent. Because analyte concentration is volume-dependent, an assay’s results might be falsely interpreted if liquid handler variability and inaccuracies are unknown or if the system(s) go unchecked for a long period.

Inkjet System for Protein Crystallography

Feb 1

X-ray crystallography is used routinely by scientists to obtain the three dimensional structure of a biological molecule of interest.Such information can be used to determine how a pharmaceutical interacts with a protein target and what changes might improve functionality. However, the crystallization of macromolecules still remains a serious hindrance in structural determination despite impressive advances in screening methods and technologies.

Attention Deficit & Hyperactivity in a Drosophila Memory Mutant

Attention Deficit & Hyperactivity in a Drosophila Memory Mutant

Nov 9 2009

Action selection is modulated by external stimuli either directly or via memory retrieval. In a constantly changing environment, animals have evolved attention-like processes to effectively filter the incoming sensory stream. These attention-like processes, in turn, are modulated by memory. The neurobiological nature of how attention, action selection and memory are inter-connected is unknown. We describe here new phenotypes of the memory mutant radish in the fruit fly Drosophila.

Molecular details of the yeast frataxin-Isu1 interaction during mitochondrial Fe-S cluster assembly.

6 hours ago

Frataxin, a conserved nuclear encoded mitochondrial protein, plays a direct role in iron-sulfur cluster biosynthesis within the ISC assembly pathway. Humans with frataxin deficiency have Friedreich's ataxia, a neurodegenerative disorder characterized by...

Palmitate-induced skeletal muscle insulin resistance does not require NF-kappaB activation.

6 hours ago

Palmitate activates the NF-kappaB pathway, and induces accumulation of lipid metabolites and insulin resistance in skeletal muscle cells. Little information is available whether and how these processes are causally related. Therefore, the objectives were to...

Altered Expression of Regulators of the Cortical Chloride Transporters NKCC1 and KCC2 in Schizophrenia.

7 hours ago

CONTEXT: Disturbances in markers of cortical gamma-aminobutyric acid neurotransmission are a common finding in schizophrenia. The nature of gamma-aminobutyric acid neurotransmission (hyperpolarizing or depolarizing) depends on the local intracellular chloride...

Stress-induced priming of glutamate synapses unmasks associative short-term plasticity.

7 hours ago

Exposure to a stressor sensitizes or 'primes' the hypothalamic-pituitary-adrenal axis to a subsequent novel stressor. The synaptic mechanisms underlying this priming, however, are not known. We found that exposing a rat to a single stressor primed glutamate...

Prokariotic Cell Collection in Denmark

Nov 6 2009

I would like to know about a prokariotic cell collection in Denmark. Is there a cell bank in this country? I need a Lactobacillus strain for a fermentation assay and this information about the bank is very helpful for me.

Request for Entries

Oct 16 2009

Ask the Experts is your chance to get the answers to questions on applications, materials, methods, processes, and technologies. Email you question to bst_web@advantagemedia.com, and the editors of Bioscience Technology will find an appropriate expert to answer it. Watch this space in the future to see the questions your colleagues are posting.          

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Video:

Viewing SureFocus Slides

Jun 11

A demonstration of SureFocus Microscope Slides in the review of AFB Smears. SureFocus Slides are a patent-pending breakthrough in tuberculosis detection, as their fluorescent staining circle remains visible during review, Fluorescence Microscopy.

Podcasts:

Allen Institute for Brain Research

Allen Institute for Brain Research

Oct 14 2009

Discussed in this interview are both the mouse brain project and the human cortex project with an emphasis on the importance of these projects to neuroscience research.