Proteomics has emerged as an interdisciplinary research field for protein identification where technology meets biology. One of the fields that has grown strongly during the last couple of years is clinical proteomics–the study of biomarkers, which are low abundant proteins present in complex biological matrices, such as serum or tissue. Successful analysis of biomarkers requires high chromatographic resolution, and high reproducibility to identify proteins, and state-of-the-art nano LC-MS/MS instrumentation that is both easy to use and robust in operation. Dionex has addressed these challenges with its third-generation nano LC instrument, the UltiMate 3000 RSLCnano system.
The heart of this new system is a binary pump with a continuous, pulse-free direct nano flow rates. The unmatched flow-pressure footprint of 800 bar column pressure is available from 20 nL/min to 50 µL/min and offers enough pump power to drive any separation. The built-in column compartment features up to two micro switching valves capable of working at pressures up to 900 bar and can be easily pulled forward or taken out without tools thus maximizing ease-of-use. A new capillary fitting system, nanoViper provides fingertight, zero-dead-volume connections to any module, valve, and column. High-resolution columns are easily connected in series using the nanoViper fitting to achieve the highest separation efficiencies possible.