Chemotaxis assay is based on the ibidi GmbH microscopy carriers, the μ-Slides. The assay allows the real time imaging of fast moving cells during the incubation with a chemoattractant. Chemotaxis is the phenomenon where single cells direct their movements according to certain chemicals in their environment. So far, the analysis of the process under real time conditions was almost impossible. ibidi scientists generated gradients in micro-structured channels in μ-Slides that allow real time imaging of the cell migration. Researchers can follow chemotactical assays on inverted microscopes. The assay is appropriate to cell systems that move with a speed of 5-10 μm per minute, like macrophages, neutrophils or Dictyostelium discoideum.