The Universal Epi-Fluorescence Reflected Light Illuminator Module for Nikon Eclipse upright microscope models provides superior fluorescence imaging and supports all types of reflected light illumination. A precision turret holds up to six easily interchangeable reflector blocks for multiple fluorescence illumination wavelengths as well as standard reflected light techniques, such as reflected brightfield, darkfield, and DIC. This feature allows the user to utilize a variety of probes at different wavelengths on multiple stained specimens. Proprietary optional excitation balancer allows continuous changing of the excitation wavelength and deciding which color to emphasize during imaging of multi-stained samples. Phosphorescent filter block identification labels allow effortless operation in dark rooms. Phosphorescent markings indicate all of the filter wavelength specifications, ensuring easy identification of the filter name and turret position index. Noise Terminator feature significantly improves the S/N ratio, blocking stray light from entering the imaging light path and adversely affecting the image. The unit features removable and centerable field and aperture diaphragms as well as several neutral density filters to provide control of the illumination to the specimen being observed. The module is versatile enough to be used with a number of Epi-reflected observation methods, including fluorescence, brightfield, darkfield, differential interference contrast, and simple polarizing techniques, separately or simultaneously with transmitted light microscopy imaging modes.