Exchanging Out TFA from Synthetic Peptide Preparations: Why it’s Important and How to do it EffectivelyNovember 4, 2015 9:02 am | by Ivona Strug and Timothy Nadler, EMD Millipore Corporation | Articles | Comments
In this case study, we show how Trifluoroacetate (TFA) salts hamper peptide analysis by FTIR spectroscopy. Subsequently, we present a detailed method by which TFA can be exchanged for HCl, with the goal of increasing the reproducibility and accuracy of peptide-based assays.
Early metastatic breast cancer cells are, strikingly, far more like stem cells than other tumor cells, says a new Nature study. They switch from a dormant (and unnoticed) stem cell-like state in primary tumors, to a more active, virulent state when kick-starting regression years later. The discovery could result in earlier detection of—and better drugs for—metastatic breast cancers.
Getting uninterrupted sleep may be more important to people’s mood than the overall amount of sleep, according to a new study from Johns Hopkins University.
Terra’s LED strips and panels are low-maintenance and energy-efficient cleanroom-compatible lights that comply with recent energy laws. The bulbs use less energy than traditional incandescent lights and produce less heat than both incandescent and fluorescent bulbs, saving on air cooling costs.
Chipotle has closed 43 of its Pacific Northwest locations in response to an E. coli outbreak health officials have connected to the Mexican food chain. About two dozen people have fallen ill and more were being tested. And at least one lawsuit has already been filed by a woman who says she became sick after eating a burrito bowl at a Vancouver, Washington, Chipotle on Oct. 21. Here are five things to know about the outbreak.
To understand the brain’s functions, scientists have for the first time quantified the entire set of proteins ‒ the proteome ‒ in the adult mouse brain. The information about which proteins and how many of them are found in the various cell types and regions has been summarized in a protein atlas.
A team has shown for the first time how one set of specialized cells survives under stress by manipulating the behavior of key immune system cells.
Researchers show how plants handle – at the molecular level – the release of ethylene, an important gaseous stress hormone that, among other functions, regulates plant growth and stimulates the fruit ripening process. The findings could pave the way to new techniques to engineer plants to produce better crops or to turn off certain genes.
The new scanner will help scientists and clinicians understand the causes and progression of dementia, and provide ways to test the effects of new treatments. Molecular changes in the brain are believed to be responsible for dementia and the scanners have the potential to link these with the brain changes that they cause – leading to new understanding and new treatments.
The U.S. death rate has been falling for decades, but researchers have detected one group in which the rates have been steadily ticking up - middle-aged white people. Suicides and deaths from drug overdose and alcohol abuse are being blamed.
Patients with glioblastoma, a type of malignant brain tumor, usually survive fewer than 15 months following diagnosis. Since there are no effective treatments for the deadly disease, researchers developed a new computational strategy to search for molecules that could be developed into glioblastoma drugs. In mouse models of human glioblastoma, one molecule they found shrank the average tumor size by half.
AMSBIO has announced the introduction of a range of Recombinant Prokaryotic Lectins (RPLs). RPLs are enhanced glycoselective bioaffinity proteins that enable efficient detection, analysis and selective isolation of glycosylated biomolecules.
Researchers believe they’re now closer to an answer about what keeps cones working and thriving, and that their findings may one day help preserve vision in patients with age-related macular degeneration and other retinal diseases.
Health officials expect the number of people sickened by an E. coli outbreak linked to Chipotle restaurants in Washington state and Oregon to grow while they investigate the cause of the infection.
Snowy white winters are likely to become a thing of the past for most Swedes. The national weather service on Monday released a new analysis of how climate change will affect the Nordic country - and it's bad news for skiing enthusiasts.