Beckman Coulter automated solutions for primary and secondary screening, proteomics and genomics include the Biomek® 2000 and Biomek FX liquid handlers, which can operate as stand-alone systems or can be integrated into SAGIAN Core Systems with a choice of other assay devices.
Advanced Tissue Arrayer (ATA-100) makes tissue microarrays from formalin-fixed, paraffin-embedded normal and tumor tissues, cell lines, xenographs and transgenic animals. A stereo microscope and movable stage provide dual viewing of the reference slide (H&E or IHC stained) and donor tissue block.
Real-time investigation of cellular and sub-cellular processes. by Paul Hoeprich, Ph.D. Figure 1: Example 203 0.5 N.A. fluorescent micrographs of HeLa cells using the EIDAQ 100 High Throughput Microscopy (HTM) system.
Cell Death Detection ELISA PLUS , a quantitative ELISA for the in vitro determination of cytoplasmic, histone-associated DNA fragments following induced cell death, is a rapid, one-step test.
Kits and reagents for optimizing apoptosis detection in particular research models include proteins and antibodies to assess expression of Bcl-2 family members, survivin and IAPs and to evaluate activity of caspases, PARP and cytochrome c.
Upstate offers a rapid and sensitive microplate-based assay for the detection of H2A.X phosphorylation. Histone H2A.
MBL International has released the 2003 Apoptosis Detection and Analysis Catalog. This resource contains nearly 3 times as many products as the 2002 version, as well as updated tables, graphics, and protocols.
Caspase selective inhibitors and substrates provide critical tools for dissecting the complex mechanisms involved in apoptosis. Q-VD-OPH, a novel compound for in vivo and in vitro applications, is an irreversible broad- spectrum caspase inhibitor.
CellTiter-Blue Cell Viability Assay, a homogeneous method for estimating the number of viable
BioVision offers assays for analyzing the early, middle, and late stages in the apoptotic cascade, and for studying apoptotic events that occur in the different areas of the cells, including the plasma membrane, cytoplasm, mitochondria, and nucleus.
Chemicon® International's ApopTag® apoptosis detection kits detect DNA fragmentation, a hallmark of apoptosis. The newest kit in the line is the ApopTag Peroxidase In Situ Oligo Ligation (ISOL) Apoptosis Detection Kit.
The M30 Apoptosense ELISA, a tool for the investigation of tumor apoptosis, is specific for apoptosis in epithelial cells and has sensitivity to detect apoptosis in less than 10,000 cells.
The CellProbe HT Caspase 3/7 Whole Cell Assay Kit enables apoptosis detection. CellProbe HT assays are whole cell based and can be automated on Beckman Coulter Biomek® liquid handling workstations.
New phosphoELISA kits offered by BioSource include Akt [pT308], Akt [pS473], Bcl-2 [pS70], CREB [pS133], [pS32], JNK [pTpY183/185], p38 MAPK [pTpY180/182], STAT1 [pY701] and others.