Living cells have emerged as indispensable tools in pharmaceutical screening and in fundamental research. Their application encompasses the evaluation of drug candidates in high-throughput screening (HTS) and ADME-tox assays for the assessment of metabolic behavior and toxicity of new compounds as well as questions emanating from basic research.
Nylon wool fiber (NWF), used for separating T-cell and B-cell lymphocytes, is available in quantities of 10g and 50g, enabling users to pack columns of any size. Wool fiber uses include removing B-cells from blood and isolating T-cells with excellent viability for routine phenotyping.
VEC Technologies, Inc. manufacturers normal cultured vascular endothelial cells and endothelial cell support products. Endothelial cells are available from various species and several different anatomic sites. Custom isolation services are also provided.
D-Tube Dialyzers dialyze sample volumes from 10 to 3000 μl. Screw-cap tubes with an integrated regenerated cellulose membrane, they are easy to load and unload. They can also be used to electroelute DNA, RNA, protein or protein-DNA complexes from agarose and PAGE gels.
Stronghold clamps, connectors and lattices are constructed of nylon with 30% glass fiber. The materials produce increased strength, reduced weight and chemical resistance properties. Surfaces are non-corroding and resistant to salts and most solvents.
The yeast shuttle vector pORF-CLONE is an intelligent method for an easy and cost-effective selection of clones containing long cDNA inserts with a correct open reading frame (ORF).
PictureFrame 2.2 camera control and image-processing software package is offered for a proprietary line of scientific grade CCD cameras for microscopy. A new graphical user interface (GUI) is included, which controls the CRI Macro-Color tunable liquid crystal RGB filter.
The Gemini C18 HPLC column achieves extended pH 1-12 stability with silica performance by using a silica manufacturing process. The extreme pH ruggedness permits method developers to perform cyclic pH testing for rapid method development.
Human and Mouse IFN ELISPOT Kits, as well as a Starter ELSPOT Kit, are available from Pierce Biotechnology under its Endogen brand.
The Metabolic Monitoring System measures oxygen consumption and carbon dioxide production in lab animals. It uses an open circuit calorimetry technique and is very flexible, making the size of the animal immaterial.
The low noise two-channel wireless data acquisition system, RatPaak, is used to monitor
The indicated cell lines were plated in 12-well plates and incubated overnight at 37 C. They were then transfected at approximately 80% confluency with 1.0 μg of a capped, polyadenylated in vitro transcript encoding firefly luciferase using the TransIT-mRNA Transfection Kit.
Hypersil GOLD LC and LC/MS columns, based on highly pure silica, are available in 3 and 5 μm sizes, and in formats including standard analytical columns, KAPPA capillary columns, DASH high-throughput LC/MS columns, and PIONEER direct-connection columns, as well as in several guard formats.
PRO Lab Plus Series equipment includes the VSN-5 Nutating Mixer, the VSM-3 Vortex Mixer, the VSOS-4 Orbital Shaker and the VSR-50 Variable Speed Rocker. A variety of accessories facilitate instrument integration into current lab procedures.