The MF10 protein separation unit simultaneously separates proteins based on size and charge. The technique employs a combination of polyacrylamide membranes with a gradation of pore sizes and electrophoretic charges through the buffer. Researchers can chose from a selection of color-coded molecular weight cut-off membranes spanning the range of proteins under investigation. By controlling membranes, buffer pH and voltage, the unit can create up to eight fractions simultaneously on samples as small as 200 microliters. 2D Polyacrylamide Membrane Separation (2D PAMS) technology allows fraction preparation, de-salting and depletion of abundant proteins, upstream of 2D gels, or MS. The method is non-destructive so the proteins retain their biological activity.
