The RC-30WA closed bath, low profile chamber is adaptable to any major microscope, upright or inverted, and features a final viewing aperture of 25 mm. The chamber bottom is beveled to permit maximum access to the bottom coverslip. For unique applications, the bath geometry, volume, and perfusion solution exchange rate may be customized by adjusting the wall height from 150 to 1,500 µm using pre-cut silicone rubber gaskets. The chamber includes a pair of resistive heater elements mounted to the chamber base for use with TC-324B and TC-344B Temperature Controllers.