With a closed-loop dynamic gas control system, the Oxystreamer maintains mixed gas concentration to within 0.2% of the selected set-point levels. Ranges cover O2 concentration from 0.1 to 99.9%; CO2 concentration from 0.1 to 20.0%; N2 concentration 0 to 100%. The two output streams of this system are useful for controlling the atmosphere in a microscope stage mount cell culture incubator/chamber and the dissolved O22 and CO2 levels in a media or perfusate. This system provides precise and variable selection of O2 and CO2 gas concentrations.