Surface enhanced Raman spectroscopy (SERS) toolkit measures materials at sub-picogram levels. Comprises of a compact, laser-safe spectrometer, a data handling software package, and proprietary Klarite substrates, the kit provides an all-in-one solution for developing trace level analysis applications. Klarite substrates enhance Raman signals by several orders of magnitude and offer high levels of reproducibility for fast, accurate detection at low detection limits. The SE1000 spectrometer includes built-in video imaging and an automated XYZ sample stage for rapid sampling and data collection.