Modiro — The PTM-Explorer automates the identification of post-translational modifications (PTMs) of proteins to provide comprehensive information while reducing analysis time. Modiro analyzes thousands of mass spectrometry profiles in parallel to identify unknown mass shifts, sequence errors and unspecific enzymatic cleavage of amino acids in high throughput. In a single step, these variances are visualized and evaluated. The analysis of many spectra in parallel, together with the analysis interface, provides a more complete characterization of the protein.