Pfenex Expression Technology is a pseudomonas-based expression system for the rapidly growing class of antibody derivative proteins. Many of these new proteins are non-native structures without evolutionarily programmed folding information, so titers are often very low. The platform for production of engineered protein molecules offers high expression levels of fully functional protein; correct folding through periplasmic targeting technology; rapid strain development with ready to clone, off-the-shelf strains; and simple, scaleable downstream processing. For high throughput applications, the system will grow at very high cell densities (30-50 OD600 units) in 96 well plates in a minimal medium. Gene discovery, protein engineering, gene expression and product manufacturing can be performed in a single host organism. Dozens of host strains can be tested in parallel for improved target protein accumulation and/or stability.