ReaX Assays contain all the components required to perform a PCR amplification assay of a selected target organism or genes, encapsulated into beads; only template DNA is required prior to running the PCR protocol. Assay beads standardize and streamline set-up, and make PCR reactions robust and reproducible. The first two assays in the ReaX range are GAPDH and 16S. Each bead is in a single dose format, pre-aliquoted into either a 96-well plate or into 8 or 12-well strips. The beads are optimized for real time PCR detection of the housekeeping gene GAPDH and can be used in gene expression studies to obtain quantification of samples of an unknown concentration. They contain all the reagents required to perform GAPDH amplification including an optimized GAPDH primer set, a GAPDH Taqman probe, reaction buffer, MgCl2, dNTPs and Hot-Start polymerase. ReaX Assay 16S beads are designed as a detection assay for bacteria by PCR amplification of 16S ribosomal RNA. Amplification is confirmed using end point PCR.
