Acolyte Biomedica Ltd.
Building 227
Porton Down Science Park
Salisbury, Wiltshire, SP4 0JQ
Website: http://www.acolytebiomedica.com
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Gram Negative Bacteria Enumeration
BacLite RapiTECT GN, based on Adenylate Kinase (AK) technology, is offered for the enumeration of gram-negative bacterial cells. Typical enumeration results can be achieved in minutes, and growth-based results within a few hours. Identified uses include rapid enumeration of bacteria in a sample for direct quantification and spiking purposes; determining growth, or inhibition of growth, of bacteria in antimicrobials; evaluating the burden of genetic manipulation on organism viability; and optimizing conditions for bacterial growth. The assay uses AK as a highly sensitive marker to detect bacterial cell numbers. AK is present at constant levels in all bacteria, irrespective of the organism’s metabolic state, and can be used to detect bacteria at low levels due to the ADPATP amplification process. The assay is based on generic gram-negative bacterial lysis and AK mediated bioluminescence. The bacteria in a sample are non-selectively lysed, releasing the AK from the bacteria into the sample matrix.
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