New version of VisiQuest software features a toolbox with 60 additional functions for image registration and segmentation tasks. Designed to save time and effort by eliminating manual steps in image registration, the new toolbox provides a variety of functions, or “glyphs,” that help solve the challenge of mapping data of a rotated image to a fixed image. The toolbox includes high performance glyphs for the registration framework components of interpolating, optimizing and transforming pixel data points. Without needing to learn a proprietary programming language, users quickly develop solutions by simply dragging-and-dropping the glyphs into the visual programming environment. For segmentation, users can tune the parameters of a combination of glyphs in an intuitive visual environment to create different methods to select areas of interest within images. Multiple glyphs for functions such as edge detection and thresholding allow easy segmentation of the image.