HTRF (Homogeneous Time-Resolved Fluorescence) Transcreener is a universal assay for high-throughput screening and profiling of kinases. This assay is a homogeneous, non-radioactive solution for kinases and ATPases. It measures the generation of ADP (Adenosine 5’-diphosphate), which is directly detected by a specific antibody labeled with Eu3+ cryptate and correlates tightly with the amount of phosphorylated substrate. By measuring ADP and not the substrate itself, the assay is compatible with any substrate. The enzyme-free detection mode also provides an alternative with less interference and a lower false positive rate than assays which measure ATP depletion. This is particularly suitable when measuring difficult kinases, such as ones where no specific antibody is available.
