CIS-US, Inc. of Cisbio International
10 DeAngelo Drive
Bedford, MA, 01730
Website: http://www.htrf-assays.com
|
 |
Kinase, Ubiquitin Assay Kits
New kinase and ubiquitin kits cover a range of targets in high throughput screening applications. The kinase toolbox, which measures functional kinase activity, encompasses the necessary technical requirements for the development of high throughput screening assays using HTRF technology. All kinase assays use a flexible assay format allowing for standard miniaturization to 384 low volume or 1536 well formats. The kit consists of Eu3+cryptate labeled anti-phospho-serine-threonine antibodies and anti-phospho-tyrosine antibodies, enabling the study of a large variety of kinases involved in cell biology. The ubiquitin kit is offered for study of the poly-ubiquitination pathway, which is acknowledged to be a significant degradation pathway for cellular proteins. Ubiquitin is a protein whose main function is to mark other proteins for proteolysis (protein breakdown) as well as marking transmembrane proteins for removal from the membrane. Both ubiquitin and the protein to be degraded are susceptible to recognition by specific enzymes that target poly-ubiquitination of the protein.
|
|
