The Cell Growth and Discovery (CGD) WorkCell is a fully enclosed, environmentally controlled automated system for high capacity cell growth, supply and in-line image analysis. Combining software with robotics, the turn-key system can simultaneously handle multiple plate/flask formats and perform cell maintenance, colony selection and RNAi studies. It has been designed for the growth and analysis of multiple cell lines and cultures. Full level II HEPA containment is offered. Strategic ports provide access while protecting cells from contamination and users from potentially hazardous aerosols and vapors. The WorkCell can perform a variety of assays at the optimal point of cell growth and has extensive process flexibility to meet the culture and experimental demands of varying cell types.