Chemotaxis slide allows the motion tracking of slow migrating cells like chemotactic active cancer cells in gradients. Up until now, comparable cell-based assays have only allowed the tracking of fast migrating cells such as neutrophiles or dictiostelia. The µ-Slide series allows the formation of long-term stable gradients over a 48 hour period. This means that one can follow the motion of slow moving cells with a speed of roughly one cell length per hour. Assays generated in this way allow cell tests in small volumes since they require only three µl cell volume and a Chemokine amount of 18 l. The slide comes with a software plug-in for Image J, which can be downloaded for free at the company’s website.