Genetix, USA
56 Roland Street
Boston, MA, 02129
Website: http://www.genetix.com
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Microarrays Screen For RNA Interference
Microarray products offered by Genetix, Inc., are suitable for screening the efficacy of short-interfering RNA (siRNA) molecules in RNA Interference (RNAi) studies. Double-stranded siRNA molecules (typically 20-25 nucleotides in length) can be introduced into mammalian cells by a variety of techniques. Once inside the cell, these molecules interact with complementary target mRNA and specific proteins to form an RNA-induced silencing complex (RISC), which reduces or ablates the level of protein expression from the gene of interest. It is usually necessary to test and optimize a variety of oligonucleotide sequences for their efficacy in silencing a given gene, which can be done in a parallel and high-throughput manner by microarraying. The synthetic siRNA duplexes or the plasmids which are eventually processed into siRNA molecules in the cell can be arrayed with a matrix of lipid transfection reagents onto a microarray slide using the QArrayMini or QArray2. The slide is then overcoated with a monolayer of adherent cells, which subsequently become transfected upon contact with the arrayed spots. In the case of an RNAi screen where the cell line is expressing a GFP fusion protein to the target gene, the efficacy of each siRNA molecule is assessed by the formation of plaques (non-fluorescent regions) in the lawn of fluorescent GFP-expressing cells. Images of GFP fusion protein expressing cells can be acquired and analysed using the Genetix aQuire scanner with optional blue laser.
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