A silica-based stationary phase that withstands temperatures up to 200 C is incorporated in the Blaze200 column. The column’s polydentate silica phase is stable at extreme temperatures, with added pH as high as 12. The development of a polycarbosilane bonding chemistry that is hydrolytically stable against breakdown in aqueous environments facilitates such analyses. It integrates multiple point attachments to the silica with a high degree of cross-linking. This combination creates a highly protective barrier over the silica backbone, protecting it from hydrolytic attack. The column offers traditional C18 selectivity and is useful for both isothermal and programmed separations.
