INTEGRA Biosciences AG
Schonbuhlstr. 8
CH - 7000 ChurWebsite: http://www.integra-biosciences.com
|
 |
Long-term high-level Protein expression
INTEGRA Biosciences (www.integra-biosciences.com) has announced a new applications study, undertaken in conjunction with Eugenix Biotechnologies (Tagerwilen, Switzerland), describing a disposable flask bioreactor for long-term cultivation of high levels of recombinant proteins. Optimized for protein expression from adherent cells, the CELLine adhere disposable flask bioreactor incorporates a woven polyethylene teraphthalate matrix in the cell compartment to provide a surface for attachment of anchorage dependent cells such as HEK, BHK and CHO. Through use of a novel membrane technology in a two-compartment bioreactor the CELLine adhere enables high cell densities and corresponding product concentrations to be grown in both batch and semi-continuous culture modes. The small volume of resultant concentrated protein product offers significant savings in the time, labor and material costs of the downstream processing and purification. The study clearly demonstrates the utility of the CELLine adhere in rapidly and cost effectively generating protein lead structures from an adherent mammalian cell line in quantities of 50-200 mg suitable for biochemical and in-vivo activity studies.
|
|
