NimbleGen Systems, Inc.
Headquarters
1 Science Court
Madison, WI, 53711
Website: http://www.nimblegen.com
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Microbial Whole Genome Resequencing Technology
NimbleGen Systems offers a new technology for rapid microbial resequencing. The ability to identify the genetic variation between a reference genome and closely related microbial isolates is important for studying antibiotic resistance, the evolution of a microbe as it spreads in the public sector, homeland security, and other applications. The method surveys an entire microbial genome to pinpoint genetic changes, down to the single nucleotide level. A two-stage approach is used to identify genomic changes. In stage one, nearly all locations where the strain’s genome deviates by even a single nucleotide are detected. In stage two, each nucleotide deviation is resequenced using microarrays to determine the new genetic code, and larger regions of insertion and deletion are also automatically flagged for further characterization using other methods. Overall false positive rate is typically less than one false call per one million bases analyzed. The company employs 385,000 probe microarrays and a novel isothermal probe selection strategy to enable consistent detection of sequence differences between genomes. The Maskless Array Synthesis (MAS) platform facilitates rapid and cost-effective production, allowing customized arrays — a key component in the second phase of the technique — that can finely tile across the regions of genomic difference.
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