Agilent Technologies, Inc.
Life Sciences & Chemical Analysis
P.O. Box 51000
Santa Clara, CA, 92055-1000
Website: http://www.agilent.com/chem
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HPLC-CHIP/MS System
The HPLC-chip/mass spectrometry (MS) system for protein identification is a microfluidics-based LC/MS technology reduces required sample sizes by integrating multiple functionalities on a single microfluidics-based chip. The Protein ID HPLC-Chip offers broad peptide coverage and high protein identification. Smaller than a credit card, the chip integrates the sample enrichment and separation capability of a nanoflow LC system with the intricate connections and spray tips used in electrospray mass spectrometry. The technology eliminates 50% of the fittings and connections typically required. A radio frequency tag contains information such as chip ID and type, documentation, diagnostic and operational data, and additional memory for user comments. The RF tag is read each time the chip is inserted into the HPLC-Chip Cube, and information is updated on the tag before chip removal. A 43 mm × 75 μm column is packed with 5 μm Zorbax Stable Bond 300A C-18 reverse phase material. The second component of the system is the HPLC-Chip/MS Cube interface, which is mounted on the proprietary Nanoflow Proteomics Solution XCT+ Ion Trap mass spectrometer. In addition to positioning the sprayer tip orthogonal to the MS inlet to maximize sensitivity and robustness, the interface automatically loads and seals the chip, establishing high pressure, leak tight fluid connections. Chip replacement is simple and can be completed in a few seconds. The cube is available as a standard module within the proprietary 1100 Series LC System for MS.
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