MetWorks 1.1.0 (Thermo Fisher Scientific) facilitates automated acquisition, processing and reporting of LC/MSn data in support of biotransformation studies. The software is compatible with all mass spectrometers capable of performing MS/MS and MSn fragmentation. It allows high resolution accurate mass data from the LTQ Orbitrap and LTQ FT instruments to be fully leveraged. Enhanced tools facilitate distinguishing xenobiotic components from endogenous biological matrix interferences in LC/MSn chromatograms and spectra.