Major software developments for the Thermo Fisher Scientific high-content screening and analysis platform include the introduction of the HCSExplorer informatics software, new functionality of the BioApplications line of image analysis software, and expanded capability of the HCS Analysis Platform. In addition, a Brightfield module for the Cellomics ArrayScan VTi provides assay flexibilityby adding white light imaging to its fluorescent imaging capabilities. HCSExplorer software uses Web-based technology to enable rapid and easy enterprise-wide data access and analysis. Bioapplications are image analysis algorithms that allow scientists to make complex measurements and are validated for a variety of biological methods. Version 3 measures larger image sizes and more objects within any given field. A number of “tool tips” such as the background correction in each channel and the ability to analyze objects on a high contrast background improves usability.