NuGEN Technologies, Inc.
821 Industrial Road, Unit A
San Carlos, CA, 94070
Website: http://www.nugeninc.com
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RNA Amplification System For Gene Expression Analysis
The Ovation RNA Amplification System (NuGEN Technologies, Inc.), enables analysis of small samples for gene expression experiments, especially those based on real-time quantitative PCR (QPCR). Use of the system prior to QPCR analysis amplifies mRNA in potentially limited, biologically important samples such as small biopsies or flow sorted cells. The kit offers a standardized 3.5-hour protocol that works equally well with large or small total RNA sample amounts, allowing experimentation from samples as small as 5 ng of total RNA. The end product of the protocol is cDNA, which can be directly measured by QPCR.
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