ExCyto PCR Competent Cells constitutively express a thermostable DNA polymerase, offering a reliable way to screen transformed colonies. The cells are transformed using any E. coli vector plate incubated overnight. The user picks colonies and adds to the provided Reaction Mix with primers, then cycles. ExCyto PCR eliminates mini-preps and the need for a purified, exogenous DNA polymerase for single colony PCR. Amplification is robust even with single-copy vectors. Amplicons up to 5 kb can be directly sequenced without purification after the reaction. ExCyto Chemically Competent and Electrocompetent Cells are available in single-transformation tubes, as well as 96-well plates for high-throughput applications.
