TransIT-HeLaMONSTER Transfection Kit maximizes transfection performance in HeLa and related cells. Reduced levels of cell damage provide physiologically relevant data; the phenotype observed is the result of the transfected gene, not transfection reagent toxicity. Transfections using the product are most effective when carried out in complete growth media, with no media change required. Optimized transfections yield efficiencies up to 60%. The kit is offered for transfection of single or multiple plasmids for gene expression studies, promoter analysis and other applications for both transient and stable transfections.