Dual Expression Vector System - Bioscience Technology Online

MoBiTec GmbH

Lotzestrasse 22a
Goettingen, D-37083
Website: http://www.mobitec.com

Dual Expression Vector System

MoBiTec's new pPICHOLI shuttle vector system has been constructed for efficient protein expression in both Escherichia coli and Pichia pastoris making subcloning experiments superfluous. While E. coli is simple to handle and enables cost-effective high-level production of recombinant proteins, the expression of many (mainly eukaryotic) genes often results in the production of aggregated and denatured proteins, accumulated in inclusion bodies. In contrast, the yeast P. pastoris is suited for synthesis of soluble heterologous target proteins with post-translational modifications. Its rapid growth at high cell density combined with a strong, inducible promotor on pPICHOLI allow for expression of recombinant proteins in high yields. Genes cloned in pPICHOLI are expressed in E. coli and P. pastoris as fusion proteins with HIS-, HA- and biotin tags enabling a simple, fast and reproducible purification via affinity chromatography and detection by commercially available specific antibodies.