The Fluoview FV1000-EVA module adds computer control of excitation laser light at or beyond the critical angle for TIRF and evanescent wave excitation. By combining the flexibility of a traditional laser scanning system with TIRF capabilities, it offers system flexibility. Scientists doing TIRF illumination can opt to use any of the numerous laser wavelengths designed for confocal illumination, including 440 nm, 488 nm, 515 nm, a new 559 nm, and other wavelengths. TIRF images are acquired on a CCD camera. This CCD system requires serial TTL trigger outputs for switching the output wavelength when doing sequential fluorescence observations. The FV1000-EVA features full computer control of the laser path so that when changing objectives during TIRF illumination, the instrument automatically realigns.